Project
Part:BBa_K1462410:Design
Designed by: Ye Peng Group: iGEM14_SCUT (2014-10-10)
pTEF2+GroES+tADH1+pTEF2+GroEL+tADH1+pGAL1+PRK+GBD-ligand+tADH1+pTDH3+RuBisCo+SH3-ligand+tADH1+pTDH3+
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4138
Illegal BglII site found at 8442
Illegal BglII site found at 9468
Illegal BamHI site found at 10286
Illegal XhoI site found at 10267 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3639
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part consists of promoter TEF2 + chaperon GroES + terminator ADH1 + promoter TEF2 + chaperon GroEL + terminator ADH1 + promoter GAL1+ PRK + the ligand of the scaffold GBD + terminator ADH1 + promoter TDH3 + RuBisCo + the ligand of the scaffold SH3 + terminator ADH1 + promoter TDH3 + CA + the ligand of the scaffold PDZ+ terminator ADH1
Source
Kits and a synthesis company.